High Pure Viral Nucleic Acid Kit from Roche

Product Specs
ItemHigh Pure Viral Nucleic Acid Kit
CompanyRoche
Price Get Pricing Get Quote for this Product
Catalog Number11858874001
Quantity1 kit (for up to 100 purifications)
SourceBlood, Plasma/Serum

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Topoisomerase Inhibitors Increase Episomal DNA Expression by Inducing the Integration of Episomal DNA in Hepatic CellsPharmaceuticsOctober 13, 2023Andoni Gómez-Moreno, Enara San Sebastian, Jennifer Moya, Pilar Gomollón-Zueco, Sergio Isola, África Vales, Gloria González-Aseguinolaza, Carmen Unzu, Urtzi GaraigortaBedford, MA, USA). AAV vector genomes were extracted using the High Pure Viral Nucleic Acid Kit (cat. nº: 11858874001, Roche, Switzerland) according to the manufacturer’s specifications. The quality of the vector production

(2) Virome Analysis and Association of Positive Coxsackievirus B Serology during Pregnancy with Congenital Heart DiseaseMicroorganismsJanuary 19, 2023Mathieu Garand, Susie S. Y. Huang, Lisa S. Goessling, Fei Wan, Donna A. Santillan, Mark K. Santillan, Anoop Brar, Todd N. Wylie, Kristine M. Wylie, Pirooz Eghtesadynucleic acid was extracted with the High Pure Viral Nucleic Acid Kit (Roche, Basel, Switzerland, catalog #11858874001) according to the manufacturer’s instructions. For co-culture experiments, the media was concentrated

(3) Subviral Dense Bodies of Human Cytomegalovirus Induce an Antiviral Type I Interferon ResponseCellsDecember 13, 2022Penner I, Büscher N, Dejung M, Freiwald A, Butter F, Plachter B.purchased from ThermoFisher Scientific, Darmstadt, Germany. The High Pure Viral Nucleic Acid Kit (#11858874001) was purchased from Roche Holding, Basel, Switzerland. 2.7. Generation of the HFF-Knockout Cells Using

(4) Recombinant Human Cytomegalovirus Expressing an Analog-Sensitive Kinase pUL97 as Novel Tool for Functional AnalysesVirusesOctober 17, 2022Krämer N, Schütz M, Mato UG, Herhaus L, Marschall M, Zimmermann C.200 µL supernatant and 1 × 105 cells by using the High Pure viral nucleic acid kit (Roche Holding AG, 11858874001) according to the manufacturer’s instructions. The quantification was performed by qPCR. 2.5. Western BLOT

(5) Insight and Development of Advanced Recombinant Adeno-Associated Virus Analysis Tools Exploiting Single-Particle Quantification by Multidimensional Droplet Digital PCRHuman Gene TherapySeptember 1, 2022Jeanette Zanker, Sara Lázaro-Petri, Daniela Hüser, Regine Heilbronn, Adrien Savyindicated, rAAV genomes were extracted with High Pure Viral Nucleic Acid Extraction Kit (Roche—no. 11858874001) following the manufacturer's instructions and eluted in elution buffer supplemented with 0.05% F-68.

(6) A parasite outbreak in notothenioid fish in an Antarctic fjordiScienceJuly 15, 2022Thomas Desvignes et al.commercial assays,Critical commercial assays ,, High Pure Viral Nucleic Acid Kit,Roche Life Science,Cat# 11858874001 TruSeq DNA Nano Kit,Illumina,Cat# 20015964 Ribo-Zero Plus rRNA Depletion Kit,Illumina,Cat# 20040526 DNeasy

(7) The Prevalence and Genetic Diversity of PCV3 and PCV2 in Colombia and PCV4 Survey during 2015–2016 and 2018–2019PathogensMay 31, 2022Diana S. Vargas-Bermudez, José Darío Mogollón, Jairo Jaime(stool and sera pools and tissues) were extracted using the High pure nucleic acid kit (Roche®, Ref 11858874001, Mannheim, Germany). PCV2, PCV3, and PCV4 were detected by conventional PCR. It is important to note

(8) Repurposing of FDA Approved Drugs Against SARS-CoV-2 Papain-Like Protease: Computational, Biochemical, and in vitro StudiesFrontiers in MicrobiologyMay 10, 2022Rajkumar Kulandaisamy et al.was processed to prepare the viral genomic RNA using HiPure™ Viral Nucleic Acid extraction kit (Cat # 11858874001; Roche Diagnostics, India). One-third of the viral genomic RNA preparation was reverse-transcribed as

(9) Comparison and efficacy of two different sheep pox vaccines prepared from the Bakırköy strain against lumpy skin disease in cattleClinical and Experimental Vaccine ResearchJanuary 31, 2022Serdar Uzar, Fahriye Sarac, Veli Gulyaz, Hakan Enul, Huseyin Yılmaz, Nuri Turanextraction by using a commercial DNA extraction kit (Roche High Pure Viral Nucleic Acid Kit, cat. no., 11858874001; Roche Diagnostics, Basel, Switzerland). Ethical approval This study was carried out in accordance

(10) Use of guanidine thiocyanate-based nucleic acid extraction buffers to inactivate poliovirus in potentially infectious materialsJournal of Virological MethodsNovember 1, 2021Michelle J. Honeywood, Stacey Jeffries-Miles, Kimberly Wong, Chelsea Harrington, Cara C. Burns, M. Steven Oberste, Michael D. Bowen, Everardo VegaMD); Quick-RNA Viral Kit (Cat No. R1035; Zymo Research); High Pure Viral Nucleic Acid Kit (Cat. No. 11858874001; Roche, Wilmington, MA); MagMAX Lysis/Binding Buffer (Cat. No. 8500; part of the MagMAX Pathogen RNA/DNA

(11) Chikungunya virus molecular evolution in India since its re-emergence in 2005Virus EvolutionSeptember 1, 2021Sakshi Chaudhary, Jaspreet Jain, Ramesh Kumar, Jatin Shrinet, Scott C Weaver, Albert J Auguste, Sujatha SunilRNA was isolated directly from patient sera using the High Pure Viral Nucleic Acid kit (Product No: 11858874001; Roche, Germany) according to the manufacturer’s instructions. The integrity of RNA was estimated using

(12) Comparison of reverse-transcriptase polymerase chain reaction (RT-PCR) and rapid test for the detection of bovine rotavirus and bovine coronavirus in anatolian water buffaloesEurasian Journal of Veterinary SciencesSeptember 1, 2020Oya Bulut, Gülşah Uyunmaz Saklı, Mustafa Hasöksüz, Irmak Dik, Hasan Hüseyin Hadimli, Mustafa Hitittwo methods. BCoV RNA extraction and RT-PCR assays The High Pure Viral RNA isolation kit (Roche. Cat. No: 11858874001, Mannheim, Germany) was used for RNA extraction. BRV Dimethyl sulfoxide (0.8 μl), BRV forward and

(13) Ex vivo model of herpes simplex virus type I dendritic and geographic keratitis using a corneal active storage machinePLOS ONEJuly 22, 2020Emilie Courrier et al.processing. Viral DNA was extracted from each cornea wedge using the high pure viral nucleic acid kit (11858874001, Roche LifeScience) with some adaptations described in the supporting information (S1 Fig). PCR was

(14) Evaluation of Chemical Protocols for Inactivating SARS-CoV-2 Infectious SamplesVirusesJune 8, 2020Boris Pastorino, Franck Touret, Magali Gilles, Lea Luciani, Xavier de Lamballerie, Remi N. Charrelthis kit. The composition of lysis buffers commercialized by ROCHE in HighPure Viral Nucleic Acid (ref#11858874001) and HighPure Viral RNA (ref#11858882001) kits contain guanidinium chloride (30%–50%), Triton-X100 (10%–20%

(15) Chikungunya Outbreaks in India: A Prospective Study Comparing Neutralization and Sequelae during Two Outbreaks in 2010 and 2016The American Journal of Tropical Medicine and HygieneApril 1, 2020Jaspreet Jain et al.RNA was isolated directly from patient sera using the High Pure Viral Nucleic Acid kit (Product No: 11858874001; Roche, Mannheim, Germany) according to the manufacturer’s instructions. Viral RNA samples were screened

(16) Molecular characterization of contagious ecthyma virus (CEV) isolated from goat and cross-protection studiesMedycyna WeterynaryjnaJanuary 1, 2020VELI GULYAZ, MUSTAFA HASOKSUZ, METING BULUT, FAHRIYE SARAÇHigh Pure Viral Nucleic Acid Kit (Roche, Cat No: incubated in an oven at 37°C in a 5% CO condition. Cells 2 11858874001) according to the protocols specified in the kit. were collected when a 90% cytopathologic effect (CPE) For

(17) Investigation of bovine coronavirus and bovine rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf fecesJournal of Istanbul Veterinary SciencesDecember 31, 2019Gülşah UYUNMAZ SAKLI, Oya BULUT, Mustafa HASÖKSÜZ, Hasan Hüseyin HADİMLİMaterials: Extraction of the Viral RNA: We used High Pure Viral RNA isolation kit of Roche (Roche, Cat. No: 11858874001). The fecal samples were suspended at a rate of 1/10 in PBS including 25000 U/ml Penicillin and 20 mg/ml

(18) HCV and flaviviruses hijack cellular mechanisms for nuclear STAT2 degradation: Up-regulation of PDLIM2 suppresses the innate immune responsePLoS pathogensAugust 1, 2019Joyce MA, Berry-Wynne KM, Dos Santos T, Addison WR, McFarlane N, Hobman T, Tyrrell DL.quantitative real-time PCR Extracellular HAV and HCV RNA were purified using the Roche High Pure Viral Nucleic Acid kit (11858874001) as per manufacturer’s instructions. cDNA was synthesized with Superscript III (ThermoFisher Scientific,

(19) Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRaMolecular Therapy - Nucleic AcidsJune 7, 2019Cia-Hin Lau, Jonathan Weng-Thim Ho, Pik Kwan Lo, Chung Tinacids from AAV vector preparations were extracted with the High Pure viral nucleic acid kit (Roche 11858874001), according to the manufacturer’s instructions. Briefly, 20 μL harvested viral supernatant was used

(20) Widespread circulation of West Nile virus, but not Zika virus in southern IranPLOS Neglected Tropical DiseasesDecember 17, 2018Mazyar Ziyaeyan, Mohammad Amin Behzadi, Victor Hugo Leyva-Grado, Kourosh Azizi, Gholamreza Pouladfar, Hedayat Dorzaban, Atoosa Ziyaeyan, Sanaz Salek, Aghyl Jaber Hashemi, Marzieh Jamalidoustof each mosquitoes’ homogenate or patient sera using the High pure Viral RNA Kit (catalogue number: 11858874001, Roche Diagnostics, Mannheim, Germany) according to the manufacturer’s instructions. Real-time reverse

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High Pure Viral Nucleic Acid Kit from Roche

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High Pure Viral Nucleic Acid Kit from Roche

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